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    Application of CRISPR/Cas for diagnosis and management of viral diseases of banana

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    Journal Article (1.526Mb)
    Date
    2021
    Author
    Tripathi, L.
    Ntui, V.O.
    Tripathi, J.N.
    Kumar, P.L.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Viral diseases are significant biotic constraints for banana (Musa spp.) production as they affect the yield and limit the international movement of germplasm. Among all the viruses known to infect banana, the banana bunchy top virus and banana streak viruses are widespread and economically damaging. The use of virus-resistant bananas is the most cost-effective option to minimize the negative impacts of viral-diseases on banana production. CRISPR/Cas-based genome editing is emerging as the most powerful tool for developing virus-resistant crop varieties in several crops, including the banana. The availability of a vigorous genetic transformation and regeneration system and a well-annotated whole-genome sequence of banana makes it a compelling candidate for genome editing. A robust CRISPR/Cas9-based genome editing of the banana has recently been established, which can be applied in developing disease-resistant varieties. Recently, the CRISPR system was exploited to detect target gene sequences using Cas9, Cas12, Cas13, and Cas14 enzymes, thereby unveiling the use of this technology for virus diagnosis. This article presents a synopsis of recent advancements and perspectives on the application of CRISPR/Cas-based genome editing for diagnosing and developing resistance against banana viruses and challenges in genome-editing of banana.
    https://dx.doi.org/10.3389/fmicb.2020.609784
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7062
    IITA Authors ORCID
    Leena Tripathihttps://orcid.org/0000-0001-5723-4981
    Ntuihttps://orcid.org/0000-0003-4709-8087
    Dr Jaindra Nath Tripathi, Ph.D.https://orcid.org/0000-0002-6366-917X
    P. Lava Kumarhttps://orcid.org/0000-0003-4388-6510
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3389/fmicb.2020.609784
    Research Themes
    Biotech and Plant Breeding; Plant Production and Health
    IITA Subjects
    Agronomy; Banana; Food Security; Plant Breeding; Plant Diseases; Plant Health; Plant Production
    Agrovoc Terms
    Bananas; Diseases; Plant Diseases; Banana Bunchy Top Virus; Genomic Selection; Disease Resistance; Viral Diseases
    Regions
    Africa; Central Africa; Southern Africa; West Africa
    Countries
    Benin (Dahomey); Cameroon; Mozambique; Nigeria; South Africa; Zambia
    Hubs
    Eastern Africa Hub; Headquarters and Western Africa Hub
    Journals
    Frontiers in Microbiology
    Collections
    • Journal and Journal Articles4835

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