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dc.contributor.authorTripathi, L.
dc.contributor.authorNtui, V.O.
dc.contributor.authorTripathi, J.N.
dc.contributor.authorKumar, P.L.
dc.date.accessioned2021-03-03T10:33:39Z
dc.date.available2021-03-03T10:33:39Z
dc.date.issued2021
dc.identifier.citationTripathi, L., Ntui, V.O., Tripathi, J.N. & Kumar, P.L. (2021). Application of CRISPR/Cas for diagnosis and management of viral diseases of banana. Frontiers in Microbiology, 11, 609784: 1-13.
dc.identifier.issn1662-302X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7062
dc.description.abstractViral diseases are significant biotic constraints for banana (Musa spp.) production as they affect the yield and limit the international movement of germplasm. Among all the viruses known to infect banana, the banana bunchy top virus and banana streak viruses are widespread and economically damaging. The use of virus-resistant bananas is the most cost-effective option to minimize the negative impacts of viral-diseases on banana production. CRISPR/Cas-based genome editing is emerging as the most powerful tool for developing virus-resistant crop varieties in several crops, including the banana. The availability of a vigorous genetic transformation and regeneration system and a well-annotated whole-genome sequence of banana makes it a compelling candidate for genome editing. A robust CRISPR/Cas9-based genome editing of the banana has recently been established, which can be applied in developing disease-resistant varieties. Recently, the CRISPR system was exploited to detect target gene sequences using Cas9, Cas12, Cas13, and Cas14 enzymes, thereby unveiling the use of this technology for virus diagnosis. This article presents a synopsis of recent advancements and perspectives on the application of CRISPR/Cas-based genome editing for diagnosing and developing resistance against banana viruses and challenges in genome-editing of banana.
dc.format.extent1-13
dc.language.isoen
dc.subjectBananas
dc.subjectDiseases
dc.subjectPlant Diseases
dc.subjectBanana Bunchy Top Virus
dc.subjectGenomic Selection
dc.subjectDisease Resistance
dc.subjectViral Diseases
dc.titleApplication of CRISPR/Cas for diagnosis and management of viral diseases of banana
dc.typeJournal Article
cg.contributor.crpMaize
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionCentral Africa
cg.coverage.regionSouthern Africa
cg.coverage.regionWest Africa
cg.coverage.countryBenin (Dahomey)
cg.coverage.countryCameroon
cg.coverage.countryMozambique
cg.coverage.countryNigeria
cg.coverage.countrySouth Africa
cg.coverage.countryZambia
cg.coverage.hubEastern Africa Hub
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.researchthemePlant Production and Health
cg.identifier.bibtexciteidTRIPATHI:2021
cg.isijournalISI Journal
cg.authorship.typesCGIAR Single Centre
cg.iitasubjectAgronomy
cg.iitasubjectBanana
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.journalFrontiers in Microbiology
cg.notesOpen Access Journal; Published online: 27 Jan 2021
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3389/fmicb.2020.609784
cg.iitaauthor.identifierLeena Tripathi: 0000-0001-5723-4981
cg.iitaauthor.identifierNtui: 0000-0003-4709-8087
cg.iitaauthor.identifierDr Jaindra Nath Tripathi, Ph.D.: 0000-0002-6366-917X
cg.iitaauthor.identifierP. Lava Kumar: 0000-0003-4388-6510
cg.futureupdate.requiredNo


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